Staff
Om Prakash


Professor
Ph. D. (BHU)
Contact Information:
Tel: +91-542-6701602, +91-542-6702589, +91-542-6702590.
Email Id:[email protected]
Employee No. 11999

Acaedemic Qualifications:
S. No.DegreeInstitutionYear
1.High SchoolU.P. Board, Allahabad 1968
2.P.U.C.Banaras Hindu University1969
3.B.Sc. (Hons) Banaras Hindu University 1972
4.M.Sc. Banaras Hindu University 1974
5.Ph.D.Banaras Hindu University 1978
Area of Specialization:

Our current area of research is enzymology and enzyme technology. In the past decade, urease from watermelon and pumpkin seeds has been isolated, purified upto electrophoretic homogeneity and extensively characterized. The purified urease has also been immobilized in alginate beads and agarose gel and successfully employed for quantitation of blood urea and heavy metals in the aqueous samples.

In addition to urease, we are also working on α-amylase which is also being purified to apparent homogeneity. Work is in progress to explore its possibility for employing it for industrial/analytical applications.

Contact Informations:
(a) Office:
Department of Biochemistry
Faculty of Science
Banaras Hindu University
Varanasi - 221005.
Tel: 0542-6701602, 6702589, 6702590.
E-mail: [email protected]
(b) Residence:
N.13/209-2A, Brij Enclave, Sunderpur, Varanasi - 221005.
Tel: 0542-2316509
Current Research Projects:Two
S. No. Name of the Project Duration Source Amount (in Rs)
1.............
2.............
 
Honours/Awards (National/ International):

  1. Chaired one of the Scientific Sessions in the section of Biochemistry and Medicinal Chemistry in 32nd Annual Convention of Chemists, held at Department of Chemistry, University of Rajasthan, Jaipur during December 26-29, 1995.

  2. Chaired one of the sessions of Invited Lecture of Prof. K.R.K. Murthy in 10th Annual Conference of the "Physiological Society of India", held at Department of Physiology, IMS, BHU during December 4-6, 1998.

  3. Awarded Honorary Fellowship of the Indian Society of Agricultural Biochemists (FISAB) at 10th Convention of the Society held at Kanpur during November 27-30, 2006.

  4. Awarded Excellence Award of the Indian Society of Agricultural Biochemists at ICBFH-2010 held at Department of Biochemistry, BHU during February 17-20, 2010.

  5. Served as Course Coordinator, 2nd Refresher course in Life Sciences (Interdisciplinary), Organised by UGC-Academic Staff College, BHU during November 19 to December 9, 2013.



Seminar/Symposia Organized :

(i) UGC sponsored workshop on Biochemical Education, during October 24-28, 1985 (member local organizing committee).

(ii) International Seminar on Structure and Function of Enzymes, during Oct. 22-25, 1986 (member local organizing committee).

(iii) 4th Convention of The Indian Society of Agricultural Biochemists and Symposium on Recent Developments in Biochemistry, during March 20-21, 1995 (member local organizing committee and also acted as Treasurer for this convention.

(iv) Organised in capacity of Organising Secretary in the Department of Biochemistry, Faculty of Science, BHU an International Conference on "Role of Biomolecules in Food Security and Health Improvement" and XI Silver Jubilee Convention of the Indian Society of Agricultural Biochemists, during February 17-20, 2010.

Membership of Academic Bodies :

(i) Life member of the "Society of Biological Chemists, India", Bangalore.

(ii) Life member of "The Indian Society of Agricultural Biochemists", Kanpur.

(iii) Life Member of the "Society for Plant Biochemistry and Biotechnology", New Delhi.

List of 10 major Publications:

  1. Om Prakash, R.K. Sharma and J. Nagchaudhuri (1979) : A study of D-glucose uptake by small intestine of rats following in vivo vinblastine treatment. Pharmacology,18, 91-94. (IF = 1.581)

  2. Om Prakash, Mahe Talat, S.H. Hasan and R.K. Pandey (2008) : Enzymatic detection of mercuric ions in ground-water from vegetable wastes by immobilizing pumpkin (Cucumis melo) urease in calcium alginate beads. Bioresource Technology, 99, 4524-4528. (IF=5.039)

  3. Mahe Talat, Om Prakash and S.H. Hasan (2009) : Enzymatic detection of As(III) in aqueous solution using alginate immobilized pumpkin urease: Optimization of process variables by response surface methodology. Bioresource Technology, 100, 4462-4467. (IF=5.039)

  4. A.K. Singh, M.N.Tiwari, G. Upadhyay, D.K.Patel, D. Singh, Om Prakash, M.P. Singh (2010) : Long-term exposure to cypermethrin induces the nigrostriatal dopaminergic neurodegeneration in adult rats: Postnatal exposure enhances the susceptibility during adulthood. Neurobiology of Aging, 33 (2): 404-415. DOI: 10.1016/j.neurobiolaging.2010.02.018. (IF=6.634)

  5. Anand K Singh, M.N. Tiwari, Anubhuti Dixit, G. Upadhyay, D.K. Patel, D. Singh, Om Prakash and M.P. Singh (2011) : Nigrostriatal proteomics of cypermethnin-induced dopaminergic neurodegeneration : Microglia activation dependent and independent regulations. Toxicological Sciences, 122(2), 526-538. DOI: 10.1093/toxsci/kfr115. (IF= 5.093)

  6. G. Srivastava, A. Dixit, S. Yadav, D.K. Patel, Om Prakash and M.P. Singh (2012) : Resveratrol potentiates cytochrome P450 2 d22-mediated neuroprotection in maneb- and paraquat-induced Parkinsonism in the mouse. Free Radical Biology and Medicine, 52(8), 1294-1306. http://dx.doi.org/10.1016/j.freeradbiomed.2012.02.005 (IF = 5.271)

  7. S. Yadav, A. Dixit, S. Agrawal, A. Singh, G. Srivastava, A.K. Singh, P.K. Srivastava, Om Prakash, M.P. Singh (2012) : Rodent models and Contemporary Molecular Techniques: Notable Feats yet Incomplete Explanations of Parkinson's Disease Pathogenesis. Molecular Neurobiology, 46, 495-512. DOI: 10.1007/s12035-012-8291-8 (IF = 6.068)

  8. A. Dixit, G. Srivastava, D. Verma, M. Mishra, P.K. Singh, Om Prakash, M.P. Singh (2013) : Minocyclin, levodopa and MnTMPyP induced changes in mitochondrial proteome profile of MPTP and maneb and paraquat mice models of Parkinson' Disease. BBA - Molecular Basis of Diseases, 1832, 1227-1240. (IF = 5.387)

  9. Garima Srivastava, Anubhuti Dixit, S. Yadav, D.K. Patel, Om Prakash and M.P. Singh (2013) : Nicotine neuroprotects against maneb- and paraquat-induced Parkinson's disease phenotype in mouse by augmenting cytocrome P450 2d22 expression. Movement disorders 28 suppl 1, 577. (Abstract) in The 17th International Congress of Parkinson's Disease and Movement Disorders, held at Sydney, Australia June 16-20, 2013. (IF= 4.558)

  10. Anubhuti Dixit, Garima Srivastava, D. Verma, M. Mishra, P.K. Singh, Om Prakash and M.P. Singh (2013) : Effect of minocycline, levodopa and MnTMPyP on maneb- and paraquat-induced Parkinson's disease phenotype : Role of Mitochondra. Movement disorders 28 suppl 1, 578. (Abstract) in The 17th International Congress of Parkinson's Disease and Movement Disorders, held at Sydney, Australia June 16-20, 2013. (IF= 4.558)

Full List of Publications:


  1. LIST OF PUBLICATIONS OF Dr. OM PRAKASH :

  2. Om Prakash, R.K. Sharma and J. Nagchaudhuri (1979) : A study of D-glucose uptake by small intestine of rats following in vivo vinblastine treatment. Pharmacology,18, 91-94. (IF = 1.581)

  3. Om Prakash, S. Das, R.K. Sharma and J. Nagchaudhuri (1979) : In vivo intestinal transport of D-glucose and D-xylose in vinblastine treated rats. Indian J Medical Research, 69, 495-498. (IF = 1.661)

  4. Om Prakash and J. Nagchaudhuri (1979) : A study of small intestinal dipeptidases of rats: effect of vinblastine treatment. Pharmacology, 18, 271-275. (IF = 1.581)

  5. Om Prakash and J. Nagchaudhuri (1979): Effect of vinblastine on in vitro utilization of D-glucose and production of L-lactate by small intestine of rats. Indian J Medical Research, 70, 669-673. (IF = 1.661)

  6. Om Prakash and J. Nagchaudhuri (1979) : A study of intestinal dipeptidases of rats: effect of in vivo mucosal exposure to vinblastine. Physiologia Bohemoslovaca, 28, 503-507.

  7. Om Prakash and J. Nagchaudhuri (1980) : Effect of vinblastine treatment on intestinal disaccharidases of rats. Indian J Medical Research, 72, 97-102. (IF = 1.661)

  8. R.N. Singh and Om Prakash (1982) : Mechanism of oxidation of hexoses by Bromamine-T. Indian J Chemistry, 21A, 616-618.

  9. Om Prakash (1991) : Vinblastine treatment on intestinal absorptive functions of rats. In "Biomembranes in Health and Disease" (A.M. Kidwai, R.K. Upreti & P.K. Ray, eds.), Today & Tomorrow's Printers and Publishers, New Delhi, vol. 2, 427-432

  10. Om Prakash (1993) : A study of L-proline uptake by small intestine of rats: effect of vinblastine treatment. J Scientific Research, 43, 79-86.

  11. Om Prakash (1995) : Vinblastine induced alteration in the absorptive capabilities of the enterocytes of small intestine of rats. J Scientific Research, 45, 39-47.

  12. Om Prakash and G. Bhushan (1997) : Isolation, purification and partial characterisation of urease from seeds of water melon (Citrullus vulgaris). J Plant Biochemistry & Biotechnology, 6, 45-47. (IF = 0.810)

  13. Om Prakash and G. Bhushan (1997) : In vivo nitrate reductase in pea leaves (Pisum sativum L.): effect of fluoride and D-glucose on the activity. J Scientific Research, 47, 145-151.

  14. V.P. Singh, S.B. Singh, Om Prakash and B. Dhar (1997) : Symbiotic effectiveness of marked rhizobial and bradyrhizobial mutants in pea, pigeonpea and mungbean. In "Current Issues in Symbiotic Nitrogen Fixation" (G.H. Elkan and R.G. Upchurch eds.), Kluwer Academic Publishers, Netherlands, 193-196.

  15. Om Prakash and G. Bhushan (1998) : Molecular asymmetry in urease of water melon (Citrullus vulgaris). J Plant Biochemistry & Biotechnology, 7, 53-55. (IF = 0.810)

  16. Om Prakash and G. Bhushan (1998) : A study of inhibition of urease from seeds of the water melon (Citrullus vulgaris). J Enzyme Inhibition, 13, 69- 77.

  17. V.P. Singh, S.B. Singh and Om Prakash (1998) : Growth characteristics of rhizobial strains in nitrogen and carbon sources. Proc National Acad Sci., (India) 68(B) II, 127-132.

  18. Om Prakash and D.K. Vishwakarma (2001) : Inhibition of urease from seeds of water melon (Citrullus vulgaris) by heavy metal ions. J Plant Biochemistry & Biotechnology, 10, 147-149. (IF = 0.810)

  19. Om Prakash and Lata S.B. Upadhyay (2003) : Effect of thiols on the activity of urease from dehusked seeds of watermelon (Citrullus vulgaris). Plant Science, 164, 189-194. (IF=2.481)

  20. Om Prakash and Lata S.B. Upadhyay (2004) : Inhibition of water melon (Citrullus vulgaris) urease by fluoride. J Plant Biochemistry & Biotechnology, 13, 61-64. (IF = 0.810)

  21. P. K. Srivastava, K. Pathak and Om Prakash (2004) : A Study of NADP+- linked isocitrate dehydrogenase from germinating mung bean (Vigna radiata). J Plant Biochemistry and Biotechnology, 13, 145-148. (IF = 0.810)

  22. Om Prakash and Lata S.B. Upadhyay (2004) : Acetohydroxamate inhibition of the activity of urease from dehusked seeds of water melon (Citrullus vulgaris). J Enzyme Inhibition and Medicinal Chemistry, 19 (4), 381-387. (IF=1.574)

  23. Om Prakash and Lata S.B. Upadhyay (2005) : Physico-chemical characterization of water-melon urease upon immobilization in alginate beads. J Plant Biochemistry & Biotechnology, 14, 209-213. (IF = 0.810)

  24. Om Prakash and Lata S.B. Upadhyay (2006) : Immobilization imparts stability to watermelon urease to work in water miscible organic media. Biotechnology and Bioprocess Engineering, 11, 140-145. (IF=1.220)

  25. Om Prakash, S. Puliga and Lata S.B. Upadhyay (2007) : Immobilization of watermelon (Citrullus vulgaris) urease in agarose gel for urea estimation. Biotechnology & Bioprocess Engineering, 12, 131-135. (IF=1.220)

  26. S.H. Hasan, K.K. Singh, Om Prakash, M. Talat and Y.S. Ho (2008) : Removal of Cr(VI) from aqueous solutions using agricultural waste �maize bran'. J Hazardous Materials, 152, 356-365. (IF= 4.331)

  27. Om Prakash, Mahe Talat, S.H. Hasan and R.K. Pandey (2008) : Enzymatic detection of mercuric ions in ground-water from vegetable wastes by immobilizing pumpkin (Cucumis melo) urease in calcium alginate beads. Bioresource Technology, 99, 4524-4528. (IF=5.039)

  28. Om Prakash, Mahe Talat, S.H. Hasan and R.K. Pandey (2008) : Factorial design for the optimization of enzymatic detection of cadmium in aqueous solution using immobilized urease from vegetable waste. Bioresource Technology, 99, 7565-7572. (IF=5.039)

  29. Om Prakash, Mahe Talat, S.H. Hasan and R.K. Pandey (2008) : Enzymatic detection of heavy metal ions in aqueous solution from vegetable wastes by immobilizing pumpkin (Cucumis melo) urease in calcium alginate beads. Biotechnology & Bioprocess Engineering, 13, 210-216. (IF=1.220)

  30. Ghanshyam Upadhyay, A.K. Singh, A. Kumar, Om Prakash and M.P. Singh (2008): Resveratrol modulates pyrogallol-induced changes in hepatic toxicity markers, xenobiotic metabolizing enzymes and oxidative stress. European J Pharmacology, 596, 146-152. (IF=2.737)

  31. Om Prakash, Mahe Talat, and S.H. Hasan (2009) : Response surface design for the optimization of enzymatic detection of mercury in aqueous solutions using immobilized urease from vegetable waste. Journal of Molecular Catalysis B: Enzymatic, 56, 265-271. (IF=2.823)

  32. Mahe Talat, Om Prakash and S.H. Hasan (2009) : Enzymatic detection of As(III) in aqueous solution using alginate immobilized pumpkin urease: Optimization of process variables by response surface methodology. Bioresource Technology, 100, 4462-4467. (IF=5.039)

  33. Ghanshyam Upadhyay, S.P. Gupta, Om Prakash and Mahendra P. Singh (2010) : Pyrogallol-mediated toxicity and natural antioxidants: Triumphs and pitfalls of preclinical findings and their translational limitations. Chemico-Biological Interactions, 183(3), 333-340. (IF=2.832)

  34. G. Upadhyay, M.N.Tiwari, Om Prakash, A. Jyoti, R. Shanker and Mahendra P. Singh (2010) : Involvement of multiple molecular events in pyrogallol-induced hepatotoxicity and silymarin-mediated protection: Evidence from gene expression profiles. Food and Chemical Toxicology, 48 (6): 1660-1670. (IF=2.602)

  35. A.K. Singh, M.N.Tiwari, G. Upadhyay, D.K.Patel, D. Singh, Om Prakash, M.P. Singh (2010) : Long-term exposure to cypermethrin induces the nigrostriatal dopaminergic neurodegeneration in adult rats: Postnatal exposure enhances the susceptibility during adulthood. Neurobiology of Aging, 33 (2): 404-415. DOI: 10.1016/j.neurobiolaging.2010.02.018. (IF=6.634)

  36. Manindra N. Tiwari, Anand K. Singh, I. Ahmad, G. Upadhyay, D. Singh, D.K. Patel, C. Singh, Om Prakash and M.P. Singh (2010) : Effects of cypermethrin on monoamine transporters, xenobiotic metabolizing enzymes and lipid peroxidation in the rat nigrostriatal system. Free Radical Res. 44 (12), 1416-1424. DOI: 10.3109/10715762.2010.512041. (IF= 2.805)

  37. Om Prakash and Nivedita Jaiswal (2010) : α-Amylase: An ideal representative of thermostable enzymes. Applied Biochemistry and Biotechnology, 160, 2401- 2414. (IF=1.879)

  38. Om Prakash and Nivedita Jaiswal (2010): α-Amylase inhibition: Some practical considerations. Current Enzyme inhibition, 6 (3), 158-163. (ISSN : 1573- 4080)

  39. Om Prakash and Nivedita Jaiswal (2010): A highly efficient and thermo-stable α-Amylase from soybean seeds. Biotechnology and Applied Biochemistry, 57(3), 105-110. (IF=1.512)

  40. Om Prakash and Nivedita Jaiswal (2011) : Immobilisation of a Thermostable α-Amylase on agarose and agar matrices and its application in starch stain removal. World Applied Sciences Journal, 13 (3), 572-577.

  41. Om Prakash, Rajesh K. Pandey, Mahe Talat and Nivedita Jaiswal (2011): Effect of Lead ion concentration on soluble and agarose entrapped pumpkin urease. Intern. J Biotechnology and Bioengineering Research, 2, 55-63.

  42. Om Prakash, Nivedita Jaiswal and R.K. Pandey (2011) : Effect of metal ions, EDTA and sulhydryl reagents on soybean amylase activity. Asian J Biochemistry, 6 (3), 282-290. DOI: 10.3923/ajb.2011.282.290

  43. Nivedita Jaiswal, Om Prakash, M.Talat, H.S. Hasan and R.K. Pandey (2011) : Application of response surface methodology for the determination of optimum reaction conditions (temperature and pH) for starch hydrolysis by α-Amylase. Asian J Biochemistry 6 (4), 357-365. DOI: 10.3923/ajb.2011.357.365

  44. Nivedita Jaiswal and Om Prakash (2011) : Immobilization of soybean α-Amylase on Gelatin and its application as a detergent additive. Asian J Biochemistry 6 (4), 337-346. DOI: 10.3923/ajb.2011.337.346

  45. Anand K Singh, M.N. Tiwari, Anubhuti Dixit, G. Upadhyay, D.K. Patel, D. Singh, Om Prakash and M.P. Singh (2011) : Nigrostriatal proteomics of cypermethnin-induced dopaminergic neurodegeneration : Microglia activation dependent and independent regulations. Toxicological Sciences, 122(2), 526-538. DOI: 10.1093/toxsci/kfr115. (IF= 5.093)

  46. Om Prakash, Rajesh K. Pandey, Mahe Talat and Nivedita Jaiswal (2011) : Fluoride Quantitation in aqueous solution by agarose immobilized pumpkin urease. Current Enzyme Inhibition, 7 (2), 130-133. (ISSN : 1573-4080)

  47. G. Srivastava, Anubhuti Dixit, Om Prakash and M.P. Singh (2011) : Tiny non-coding RNAs in Perkinson's disease: Implications, expectations and hypes. Neurochemistry International, 59 (6), 759-769. DOI: 10.1016/j.neuint.2011.07.009 (IF = 3.601).

  48. Anand K. Singh, M. N. Tiwari, Om Prakash and Mahendra P. Singh (2012) : A current review of cypermethrin-induced neurotoxicity and nigrostriatal dopaminergic neurodegeneration. Current Neuropharmacology, 10, 64-71. (IF = 2.783 )

  49. G. Srivastava, A. Dixit, S. Yadav, D.K. Patel, Om Prakash and M.P. Singh (2012) : Resveratrol potentiates cytochrome P450 2 d22-mediated neuroprotection in maneb- and paraquat-induced Parkinsonism in the mouse. Free Radical Biology and Medicine, 52(8), 1294-1306. http://dx.doi.org/10.1016/j.freeradbiomed.2012.02.005 (IF = 5.271)

  50. Om Prakash, Jagdish Singh and Ajeet Kumar Rai (2012) : Effect of TNBS and alkali metal salts on the activity of Myrosinase from cauliflower seedlings (Brassica oleracea) var Botrytis. Environment & Ecology, 30 (3B), 961-966.

  51. M.N. Tiwari, Anand K. Singh, S. Agrawal, Satya P. Gupta, A. Jyoti, Rishi Sankar, Om Prakash, and M.P. Singh (2012) : Cypermethrin alters the expression profile of mRNAs in adult rat striatum: a putative mechanism of post-natal pre-exposure followed by adulthood re-exposure enhances neurodegeneration. Neurotoxicity Research, 22, 321-334. (IF = 3.514)

  52. N. Jaiswal, Om Prakash, M. Talat, S.H. Hasan and Rajesh K. Pandey (2012) : α-Amylase immobilization on gelatin: Optimization of process variables. Journal of Genetic Engineering and Biotechnology, 10 (1), 161-167. http://dx.doi.org/10.1016/j.jgeb.2012.03.003

  53. S. Yadav, A. Dixit, S. Agrawal, A. Singh, G. Srivastava, A.K. Singh, P.K. Srivastava, Om Prakash, M.P. Singh (2012) : Rodent models and Contemporary Molecular Techniques: Notable Feats yet Incomplete Explanations of Parkinson's Disease Pathogenesis. Molecular Neurobiology, 46, 495-512. DOI: 10.1007/s12035-012-8291-8 (IF = 6.068)

  54. A. Dixit, G. Srivastava, D. Verma, M. Mishra, P.K. Singh, Om Prakash, M.P. Singh (2013) : Minocyclin, levodopa and MnTMPyP induced changes in mitochondrial proteome profile of MPTP and maneb and paraquat mice models of Parkinson' Disease. BBA - Molecular Basis of Diseases, 1832, 1227-1240. (IF = 5.387)

  55. Om Prakash, Ajeet Kumar Rai, Jagdish Singh and P.M. Singh (2013) : Effect of heavy metal ions and carbohydrates on the activity of Cauliflower (Brassica oleracea Var. botrytis) Myrosinase. Journal of Stress Physiology and Biochemistry, 9 (2), 108-117. (IF = 1.711)

  56. Ajeet K. Rai, Om Prakash, J. Singh and P.M. Singh (2013) : Immobilization of cauliflower myrosinase on agar agar matrix and its application with various effectors. Advances in Biochemistry, 1(3), 51-56. doi: 10.11648/j.ab.20130103.12

  57. Nivedita Jaiswal and Om Prakash (2013) : An organic solvent and surfactant stable α-Amylase from soybean seeds. Acta Biochimica Polonica 60 (3), 387-393. (IF = 1.49).

  58. Garima Srivastava, Anubhuti Dixit, S. Yadav, D.K. Patel, Om Prakash and M.P. Singh (2013) : Nicotine neuroprotects against maneb- and paraquat-induced Parkinson's disease phenotype in mouse by augmenting cytocrome P450 2d22 expression. Movement disorders 28 suppl 1, 577. (Abstract) in The 17th International Congress of Parkinson's Disease and Movement Disorders, held at Sydney, Australia June 16-20, 2013. (IF= 4.558)

  59. Anubhuti Dixit, Garima Srivastava, D. Verma, M. Mishra, P.K. Singh, Om Prakash and M.P. Singh (2013) : Effect of minocycline, levodopa and MnTMPyP on maneb- and paraquat-induced Parkinson's disease phenotype : Role of Mitochondra. Movement disorders 28 suppl 1, 578. (Abstract) in The 17th International Congress of Parkinson's Disease and Movement Disorders, held at Sydney, Australia June 16-20, 2013. (IF= 4.558)

  60. Om Prakash, Ajeet Kumar Rai, J. Singh and P.M. Singh (2013) : Partial purification and kinetic properties of Myrosinase from Cauliflower (Brassica oleracea var. botrytis). Indian J Agric Biochemistry, 26(2), 190-194.

  61. Pratibha Tripathi, Ashish Singh, S. Agrawal, Om Prakash and M.P. Singh (2014) : Cypermethrin alters the status of oxidative stress in the peripheral blood: relevance to Parkinsonism. Journal of Physiology and Biochemistry 10; DOI: 10.1007/s13105-014-0359-7. Book:

  62. Mahe Talat and Om Prakash (2011) : Urease from pumpkin seeds used for the detection of hazardous metals : Urease purification, characterization and its application. LAP LAMBERT Academic publishing GmbH & Co, KG, Saarbrucken, Germany. ISBN: 978-3-8465-3229-4.

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