(a) Defining molecular targets for anticancer agents in in vivo tumor models
We have (a) characterized > 4 novel Ru (II) complexes and fisetin & emodin as modulators of Akt-COX2-EP1-apoptosis and Akt-HI1α-bioenergetic-apoptotic pathways vis a vis regression of Dalton�s lymphoma and hepatocellular carcinoma in vivo
(b) Neurobiology of Hepatic-encephalopathy (HE):
We have developed CLF model of HE and demonstrated involvement of glutamate-NMDAR-nNOS pathway in the cortex and cerebellum during pathogenesis of chronic HE. Glutamate-NMDAR-pannexin-1 & NMDAR-SIRT-1 signaling is the current focus of research in this area.

Awards
- AMBO fellowship, 2000
- BOYSCAST Fellowship of DST, 2002,
- Senior Scientist Medal of ICCB, 2009
- Member, Editorial/Reviewer Board of Scientific Journals: 03
(a) Journal of Paediatric Neurology
(b) AJBR (African Journal of Biochemistry Research)
(c) Scientific Journals International (SJI)
- Elected/Nominated for higher academic bodies of Istitutions/Scientific organizations :
(b) Member, Advisory Board: IBAS (Inst. Biotech. & Allied sciences), Sikar : 2009
(c) Served as Secretary and Executive council member of AGI
(d) Member, Executive Council: Association of Gerontology (India): 2009
(e) Member, Advisory Board: SNT Memorial Research Foundation: 2005-0nward
(f) Member advisory Board, Ranchi University, 2009
(g) Chaired Scientific Sessions in many National and International conferences
(h) Presented invited & plenary lectures
(i) Serving mentor for DST - INSPIRE programmes.
(j) Serving as Chairman, UACC, Institute of Science BHU; Chairman, ARS, BHU
(k) Served as Student Advisor, faculty of Science, BHU
(l) Served as Chairman, Student Council Election-2014, BHU
(m) Chaired scientific sessions and presented Plenary/Invited Lectures in many national and international Conferences

List of 10 best Publications:

1. Khanna A, Trigun SK (2016) Resveratrol normalizes hyperammonemia induced pro-inflammatory and pro-apoptotic conditions in rat brain. International Journal of Complementary & Alternative Medicine (in press)
2. Singh S, Mondal P, Trigun SK (2014) Acute Liver Failure in Rats Activates Glutamine- Glutamate Cycle but Declines Antioxidant Enzymes to Induce Oxidative Stress in Cerebral Cortex and Cerebellum. PLoS ONE 9(4): e95855. doi:10.1371/journal.pone.0095855
3. Mondal P, Trigun SK (2014) Pannexin1 as a novel cerebral target in pathogenesis of hepatic encephalopathy. Metabolic Brain Diseases, 29: 1007-1015DOI: 10.1007/s11011-014-9556-x
4. Singh S, Trigun SK (2014) Low grade cirrhosis induces cognitive impairment and motor dysfunction in rats: Could be a model for minimal hepatic encephalopathy. Neurosci. Lett. 559:136-140
5. Mehrotra A and Trigun SK. (2012) Moderate grade Hyperammonemia induced concordant activation of antioxidant enzymes is associated with prevention of oxidative stress in brain slices. Neurochemical Research, 37: 171-181.
6. Singh S, Trigun SK (2010) Activation of neuronal nitric oxide synthase in cerebellum of chronic hepatic encephalopathy rats is associated with up-regulation of NADPH- producing pathway. Cerebellum, 9:384-397.
7. Maurya BK, Trigun SK (2016) Fisetin modulates antioxidant enzymes and inflammatory factors to inhibit aflatoxin-B1 induced hepatocellular carcinoma in rats. Oxidative medicine and Cellular Lonevity ID 174839
8. Koiri RK, Trigun SK, Mishra L (2015) Activation of p53 mediated glycolytic inhibition-oxidative stress-apoptosis pathway in Dalton�s lymphoma by a ruthenium (II)-complex containing 4-carboxy N-ethylbenzamide. Biochimie, 110: 52-61.
9. Singh KB, Maurya BK, Trigun SK (2015) Activation of oxidative stress and inflammatory factors could account for histopathological progression of Aflatoxin- B1induced hepatocarcinogenesis in rats. Moecular and Cellular Biochemistry, 401:185-196.
10. Koiri RK, Trigun SK, Mishra L, Pandey K, Dixit D, Dubey SK (2009) Regression of Dalton�s lymphoma in vivo via decline in lactate dehydrogenase and induction of apoptosis by a Ruthenium (II)-complex containing 4-carboxy N-ethylbenzamide as ligand. Investigational New Drugs, 27: 503-516.

A. Full List of the Publications:

1. Khanna A, Trigun SK (2016) Resveratrol normalizes hyperammonemia induced pro-inflammatory and pro-apoptotic conditions in rat brain. IJCAM (Int. J. Complement Alt Med) (In press).
2. Maurya BK, Trigun SK (2016) Fisetin modulates antioxidant enzymes and inflammatory factors to inhibit aflatoxin-B1 induced hepatocellular carcinoma in rats. Oxidative medicine and Cellular Lonevity ID 174839
3. Mondal P, Trigun SK (2015) Bacopa monnieri extract (CDRI-08) modulates the NMDAR receptor subunits and nNOS-apoptosis axis in cerebellum of hepatic encephalopathy rats. ECAM: Evidence-based Complementary and Alternative Medicine, ID: 535013
4. Koiri RK, Trigun SK, Mishra L (2015) Activation of p53 mediated glycolytic inhibition-oxidative stress-apoptosis pathway in Dalton�s lymphoma by a ruthenium (II)-complex containing 4-carboxy N-ethylbenzamide. Biochimie, 110: 52-61
5. Singh KB, Maurya BK, Trigun SK (2015) Activation of oxidative stress and inflammatory factors could account for histopathological progression of Aflatoxin-B1induced hepatocarcinogenesis in rats. Mol. Cell Biochem. 401:185-196.
6. Kumar A, Gupta RK, Kumar A, Paitandi RP, Singh KB, Trigun SK, Hundal MS, Pandey DS (2015) Cataioic Ru(II), Rh(III) and Ir(III) complexes containing cyclic perimeter and 2- aminophenyl benzimidazole ligands: Synthesis, molecular structure, DNA and protein binding, cytotoxicity and anticancer activity. J. Organometalic Chemistry
7. Singh S, Mondal P, Trigun SK (2014) Acute Liver Failure in Rats Activates Glutamine-Glutamate Cycle but Declines Antioxidant Enzymes to Induce Oxidative Stress in Cerebral Cortex and Cerebellum. PLoS ONE 9(4): e95855.doi:10.1371/journal.pone.0095855
8. Mondal P, Trigun SK (2014) Pannexin1 as a novel cerebral target in pathogenesis of hepatic encephalopathy. Metabolic Brain Diseases, 29: 1007-1015DOI: 10.1007/s11011-014-9556-x
9. Singh S, Trigun SK (2014) Low grade cirrhosis induces cognitive impairment and motor dysfunction in rats: Could be a model for minimal hepatic encephalopathy. Neurosci. Lett. 559:136-140
10. Mehrotra A and Trigun SK (2013) Moderate grade hyperammonemia activates lactate dehydrogenase-4 and 6-phosphofructo-2-kinase to support increased lactate turnover in the brain slices. Mol Cell Biochem. DOI: 10.1007/s11010-013-1698-3.
11. Koiri RK, Mehrotra A, Trigun SK (2013) Targetting cancer with Ru(III/II)- phosphodiesterase inhibitor adducts: A novel approach in the treatment of cancer. Medical. Hypothesis. 80: 841-846
12. Mehrotra A and Trigun SK. (2012) Moderate grade Hyperammonemia induced concordant activation of antioxidant enzymes is associated with prevention of Oxidative stress in brain slices. Neurochemical Research. 37: 171-181
13. Singh KB, Trigun SK (2012) Apoptosis of Dalton�s lymphoma due to in vivo treatment with emodin is associated with modulations of hydrogen peroxide metabolizing antioxidant enzymes. Cell Biochem. Biophys. DOI 10.1007/s12013-011-9305-2.
14. Koiri RK, Trigun SK (2011) Dimethyl sulphooxide activates tumor nectrosis factorα-p53 mediated apoptosis and down regulates D-fructose-6-phosphate-2-kinase and lactate dehydrogenase-5 in Dalton�s lymphoma in vivo. Leuk. Res. 35: 950-956
15. Dixit N, Koiri RK, Maurya BK, Trigun SK, H�obartner C, Mishra L (2011) One pot synthesis of Cu(II) 2,2�-bipyridyl complexes of 5-hydroxy-hydurilic acid and alloxanic acid: Synthesis, crystal structure, chemical nuclease activity and cytotoxicity. J. Inorg. Biochem. 105: 256- 267.
16. Kumari N, Maurya BK, Koiri RK, Trigun SK, Saripella S, Coogan MP and Mishra L. (2011) Cytotoxic activity, cell imaging and photocleavage of DNA induced by a Pt (II) Cyclophane bearing 1,2 diamino ethane as terminal ligand. Med.Chem.Commun.2, 1208- 1216.
17. Singh S, Trigun SK (2010) Activation of neuronal nitric oxide synthase in cerebellum of chronic hepatic encephalopathy rats is associated with up-regulation of NADPH- producing pathway. Cerebellum, 9:384-397
18. Prajapati R, Dubey SK, Gaur R, Koiri RK, Maurya BK, Trigun SK, Mishra L (2010) Structural characterization and cytotoxicity studies of Ruthenium(II)-dmso-chloro complexes of chalcone and flavone derivatives. Polyhedron, 29: 1055-1061
19. Koiri RK, Trigun SK, Mishra L, Pandey K, Dixit D, Dubey SK (2009) Regression of Dalton�s lymphoma in vivo via decline in lactate dehydrogenase and induction of apoptosis by a Ruthenium (II)-complex containing 4-carboxy N-ethylbenzamide as ligand. Invest New Drugs, 27: 503-516
20. Singh S, Koiri RK, Trigun SK (2008) Acute and chronic hyperammonemia modulate antioxidant enzymes differently in cerebral cortex and cerebellum. Neurochem. Res. 33: 103-113
21. Koiri RK, Trigun SK., Dubey SK, Mishra L (2008), Metal Cu(II), Zn(II) Bipyridyls as inhibitors of Lactate dehyrogenase. BioMetals 21: 117-126
22. Shukla VK, Goel S, Trigun SK, Sharma D (2008) Electrophoretic pattern of proteins in carcinoma of the gallbladder. Eur. J. Cancer Prevention. 17: 9-12
23. Trigun S.K, Koiri R, Mishra L, Dubey S.K, Singh S, Pandey P. (2007)Ruthenium commplexes as Enzyme modulator: Modulation of Lactate Dehydrogenase by a Ruthenium (II) complex containing 4-carboxyl N-ethylbenzamide as ligand, Curent Enzyme Inhibition. 3: 243-253.
24. Singh S, Trigun SK (2007) Differential Expression of Antioxidant and Nitrosative stress enzymes in cerebral cortex and cerebellum of rats with chronic liver cirrhosis (Ab). Ann. Neurosci. 14: 107
25. Trigun SK., Singh AP, Asthana RK, Pandey SM., Pandey , P, Singh, SK., Singh, S P (2006) Assesment of Bioactivity of Fischerella species colonizing Azadirechta indica (neem) bark. Appl. Ecol. Envirn. Res. 4: 119-128
26. Trigun S.K, Pandey P, Singh S, Koiri R.K (2006) Brain metabolism adapts positively against most of the unphysiological challenges (Ab), Ann. Neurosci. 13: 47.
27. Pandey P, Singh S.K, Trigun S.K (2005) Fructose-2,6-bisphosphate associated regulatory enzymes develop in concordance in mice brain during early postnatal life. Neurol. Psych. Brain Res. 12: 69-74.
28. Pandey P, Singh SK, Trigun SK (2005) Developing brain of moderately hypothyroid Mice shows adaptive changes in the key enzymes of glucose metabolism. Neurol. Psych. Brain Res. 12: 159-164.
29. Mishra L, Singh A.K, Trigun S.K, Singh S.K and Pandey S.M (2004) Anti HIV and cytotoxic Ruthenium (II) complexes containing flavones : Biochemical evaluation in mice. Ind. J. Exptl. Biol 42: 660-666
30. Srivastava A.S, Trigun S.K. and Singh S.N. (1997) Activity of cytosolic and mitochondrial Alanine aminotransferase during pre-spawning phase of air- breathing and non-air-Breathing fresh water fishes. J. Scientific. Res. 47, 163-171.
31. Tamaru T, Trigun S.K, Okada M. and Nakagawa H. (1993) Identification of cells Expressing a D type G1 cycline in mature brain: Implication for the role in neuronal functions. Neuroscience Lett. 153, 169-172.
32. Trigun S.K. and Singh S.N. (1989) Evidence for tissue specific alterations in Zn2+- induced conformational changes in fructose-bisphosphatase of senescent rats. J. Inorg. Biochem. 35, 255-266.
33. Trigun S.K. and Singh S.N. (1989) Age-linked alterations in fructose-2,6-bisphosphate induced modulations of rat muscle phosphofructokinase. Biochem. Intl. 19, 1213- 1221. IUBMB Life
34. Trigun S.K. and Singh S.N. (1988) Comparison in the molecular properties of muscle phosphofructokinase of young and old rats. Cell. Molec. Biol. 34, 207-213.
35. Trigun S.K. and Singh S.N. (1988) Changes in the modulations of kinetics and allosteric properties of muscle phosphofructokinase of young and old rats. Arch. Gerontol. Geriatr. 7, 239- 247.
36. Trigun S.K. and Singh S.N.(1988) Influence of age on the conformation of rat muscle phosphofructokinase: Analysis of pH-dependent kinetics and limited proteolysis of the enzyme. Ind. J. Exptl. Biol. 26, 532- 534
37. Trigun S.K. and Singh S.N. (1987) Purification and age-related comparison in molecular properties of unproteolysed fructose-1,6-bisphosphatase of skeletal muscle of young and old rats. Biochem. Arch. 3, 343-351.
38. Trigun S.K. and Singh S.N. (1987) Alterations in the sensitivity to endogenous proteolysis due to storage of fructose-1,6-bisphosphatase extracts of liver and muscle of young and old rats. Compr. Gerontol. 1, 87-90.
39. Trigun S.K. and Singh S.N. (1987) In vitro studies on regulatory properties of muscle phosphofructokinase of young and old rats. Biochem. Arch. 3, 477-484.
40. Trigun S.K. and Singh S.N. (1987) Differential Expressions of Phosphofructokinase isozymes in different tissues of young and old rats. Cell. Molec. Biol. 33, 767-774.
    B. Book Chapter:
    1. Trigun S.K and Singh S.N. (1990) Indication of age-dependent conformational alterations in rat muscle PFK : Analysis by reversible oxidation of the enzyme. In. Perspectives in aging research. (Eds. Singh, R, & Singhal, G.S. ) Today & Tomorrow Printers and Publishers, New Delhi. Pp. 81-85 .
    2. Singh S.N. and Trigun S.K. (1991) Age-related alterations and modulations of cyytosolic Enzymes of liver of rats. In. Liver and Aging (Ed. Kitani, K.), Elsevier Science Publ. BV, Amsterdam, p. 253-262.
    3. Srivastava, A.S., Trigun, S.K. and Singh, S.N. (2000) Activity of cytosolic and mitochondrial Aspartate aminotransferase during pre-spawning phase of air- breathing and non-air-Breathing fishes. In. Chronobiology : (Ed. M. Shedpure), Univ. Press, Raipur, pp. 99-109.